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Maria Yu. Obolens'ka

Professor, Dr. Sci. (Mol. Biol.)
Tel: (380-44) 526-31-39;
Fax: (380-44) 526-07-59;
E-mail: 
m_obolenska@gmail.com


Research Areas:

Bioinformatics:

  • Whole genome search for target genes of transcription factors

  • Reconstruction of gene regulatory networks

  • Modeling of metabolic pathways

Gene expression and its regulation in eukaryotic cells

Folate-related metabolism of one-carbon units


Current research activities:

Systemic analysis of non-canonical functions of interferon alpha:

  • In silico genome-wide search for target genes of interferon alpha

  • Time-scale experiments on gene expression profiling in rat hepatocytes treated with interferon alpha (oligonucleotide microarray)

  • Interrelation between ligand and receptor – evidence from interferon alpha and expression of its receptor

  • Study of pathways of intracellular signal transmission from interferon alpha

  • Analysis of molecular background for side-effects of interferon alpha from central nervous system

Gene expression in the course of liver cells transition from quiescence to proliferation in regenerating rat liver:

  • Expression of interferon alpha target genes in the course of liver regeneration

  • Functioning of 2′,5′-oligo(A)synthetase–RNAse L system in the absence of viral damage and its role in the change in the program of cells

  • Regulation of interferon alpha target genes at post-transcriptional level

Reconstruction of gene regulatory network:

  • Elaboration of algorithms for reconstruction of gene regulatory network

  • Development of ensemble method with high grade of parallelization for the gene regulatory network modeling in Grid-environment

Folate-related metabolism of one-carbon units in human placenta in health and disease

  • The modeling of physiologically relevant and impaired folate-related metabolism in human placenta

  • Investigation of the impact of pathologically relevant concentrations of homocysteine on amino acids metabolism, proliferation and apoptosis in human placenta

  • Expression of genes, coding enzymes of folate-related metabolism in human placenta in the course of healthy and preeclamptic pregnancies

  • Elaboration of ex vivo system (placenta explants) for the investigation of the effect of different factors on organism functioning